Mutations affecting agonist sensitivity of the nicotinic acetylcholine receptor

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nicotinic receptor influences agonist sensitivity

From 1 School of Life Sciences, Oxford Brookes University, Oxford, OX3 0BP, UK; 2 Leibniz-Institut für Molekulare Pharmakologie & Neurocure Initiative Charité Universitäts Medizin, 13125 Berlin, Germany; 3 Structural Bioinformatics and Computational Biochemistry, Department of Biochemistry, University of Oxford, Oxford OX1 3QU; 4 Department of Pharmacology, University College London, London WC1...

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Covalent modification of engineered cysteines in the nicotinic acetylcholine receptor agonist-binding domain inhibits receptor activation.

We constructed and characterized a series of nicotinic receptor mutants with a cysteine substituted for one of the amino acid residues in the alpha-subunit between positions 183 and 198. This region of the receptor is known to participate in agonist binding and channel activation. The goal of this 'cysteine scanning mutagenesis' is to introduce the reactivity of a free thiol group into function...

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Both CY- and ,&subunits Contribute to the Agonist Sensitivity of Neuronal Nicotinic Acetylcholine Receptors

A family of genes has been identified that encodes subunits of nicotinic acetylcholine receptors (nAChRs) and is expressed in the nervous system. Functional neuronal nAChRs can be expressed in Xenopus oocytes by injection of RNA encoding 1 of 2 different &subunits (/32,84) in pairwise combination with RNA encoding 1 of 3 different a-subunits (a2, (~3, a4). We examined the sensitivity of these 6...

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Unraveling the high- and low-sensitivity agonist responses of nicotinic acetylcholine receptors.

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ژورنال

عنوان ژورنال: Biophysical Journal

سال: 1991

ISSN: 0006-3495

DOI: 10.1016/s0006-3495(91)82102-6